The next task is to delete all data after base 800. Scroll across to the right to see all of the data. Now the alignment explorer window will be open with all eight sequences included.

Hit the fifth button from the left on the toolbar to add the sequence to the alignment explorer. What do you notice about sequence .1? What do the n’s mean (at bases 58 and 59)? Look at the chromatogram. Scroll across and observe how the peaks and colors change. This the raw data that we obtain from sequencing pcr reactions. Go to the directory which has the chromatograms you downloaded and select all (select one, then hit control-a) (there should be eight. Within Mega select Align > edit/view sequencer files (Trace). This exercise is written for version 6 of MEGA, but the steps involved are almost identical in earlier versions.įirst download Mega 6, install it, then download the data files (seqs.zip) and unzip them. We will also look at how patterns in the data are reflected in the results in the trees. The goal here is to give you some exposure to raw sequence data, it’s assembly and some basic aspects of how to analyze data. Sequence data exploration and analysis using MEGA Sequence data exploration and analysis using MEGA

How to use megasync